Celebrating an important milestone in our lab's development - our first R01 grant! We are happy, proud, and excited to start a new project in collaboration with the labs of François St-Pierre and Jeff Noebels to better understand how short-term changes in inhibitory synapse strength contribute to seizure generation in epilepsy. With this project, we will use a combination of 2-photon voltage imaging and optogenetics to directly measure inhibitory postsynaptic potentials during spontaneous...

It was a pleasure to work with the team led by Francois St-Pierre on this collaborative project. We describe new genetically encoded voltage indicators, JEDI3sub and JEDI3hyp, with enhanced performance for detecting subthreshold voltage responses in neurons. These new sensors can be used with various microscopy methods, including resonant scanner 2P imaging. Mate and Shanii in the lab used JEDI3hyp to image subthreshold depolarization and hyperpolarization in PV interneurons during sharp-wave...